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为探究循环率对循环水凡纳滨对虾养殖系统水质和细菌群落组成结构的影响，分别设置10次/d(低循环率，LC)、15次/d(中循环率，MC)、20次/d(高循环率，HC)3个不同水循环率的试验系统并连续运行33 d,期间监测并分析凡纳滨对虾生长、养殖系统水质和细菌群落变化情况。结果表明，各试验组凡纳滨对虾的生长无明显差异。LC,MC和HC组亚硝酸盐氮最高质量浓度分别为2.48,2.07,1.89 mg·L-1,高循环率条件下的亚硝酸盐氮去除效率更高。菌群多样性随循环率的升高而降低。LC,MC和HC组对虾的存活率分别为61%,73.6%和79%。变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)为生物滤池中两大优势菌群，LC,MC和HC组中变形菌门相对丰度占比分别为32.34%,36.91%和38.44%,变形菌门和拟杆菌门占比随循环率的升高而升高。生物滤池中起硝化作用的细菌主要是亚硝化单胞菌属(Nitrosomonas)、硝化球菌属(Nitrococcus)和硝化螺旋菌属(Nitrospira)。高循环率的循环水系统水质更好，对虾存活率更高。
Abstract：In order to investigate effects of circulation rates on water quality and bacterial community structure of a recirculating aquaculture system for Litopenaeus vannamei, three different water circulation rates, LC(daily circulation rate of 10), MC(daily circulation rate of 15) and HC(daily circulation rate of 20), were set and run for 33 days. The changes of shrimp growth performance, water quality and bacterial community composition were analyzed during the study period. The results showed that the highest concentrations of LC, MC, and HC were 2.48, 2.07, and 1.89 mg·L-1, respectively, during shrimp recirculating water aquaculture. The study demonstrated that the removal efficiency of the system for nitrite concentration was accelerated with the increase of the water recirculation rate. The diversity of biofilm microorganisms decreased with the increase of water circulation rate. The survival rates of shrimp in LC, MC and HC groups were 61%, 73.6% and 79%, respectively. Proteobacteria and Bacteroidetes were the two dominant phylum in the experimental systems, and the relative abundance of Proteobacteria in LC, MC, and HC groups was 32.34%, 36.91%, and 38.44%, respectively. The proportion of Proteobacteria and Bacteroidetes increased with the increase of circulation rate. The nitrifying bacteria were mainly Nitrosomonas, Nitrococcus, and Nitrospira. A high recirculation rate water recirculation system was better for water quality and shrimp survival.
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基本信息:
DOI：
中图分类号:S968.22
引用信息:
[1]翟玮,孔祥青,陈钊等.循环率对循环水凡纳滨对虾养殖系统水质和细菌群落组成的影响[J].江苏海洋大学学报(自然科学版),2024,33(04):1-8.
基金信息:
国家重点研发计划专项课题(2023YFD2401704); 国家虾蟹产业技术体系项目(CARS-48); 中国水产科学研究院基本科研业务费项目(2023TD50)